Modulation of phosphorylation and dephosphorylation of keratin and other polypeptides by estradiol‐17β in rat vaginal epithelium

FEBS Letters

Swarup, G.¹  Reddy, A.G.¹  Gupta, P.D.¹ 

[1] Centre for Cellular and Molecular Biology, Hyderabad 500 007, India

关键词: Phosphorylation;    Dephosphorylation;    Estradiol;    Keratin;    Vaginal epithelium;    VEC;    vaginal epithelial cells;    if;    intermediate filament;    DMEM;    dulbecco's modified Eagle's medium;    SDS-PAGE;    sodium dodecyl sulphate polyacrylamide gel electrophoresis;    CPM;    counts per minute;   

DOI  :  10.1016/0014-5793(90)81068-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Phosphorylation of keratin polypeptides was studied by incubating vaginal tissues (removed from estradiol primed and unprimed 30-day-old rats) with ³²P_i. Analysis by SDS-PAGE and autoradiography showed that on treatment with estradiol phosphorylation of 63 and 58 kDa keratin polypeptides increased 3- and 2-fold respectively. Phosphorylation was maximal after 30 min of estradiol priming and decreased thereafter. Phosphorylation of some non-keratin polypeptides (37, 34, 32 and 25 kDa) also showed time dependent variation. The results showed that estradiol can modulate phosphorylation-dephosphorylation of keratins and other polypeptides in rat vaginal epithelial cells.

【 授权许可】

Unknown