Release of the component of Streptococcus faecalis Na+‐ATPase from the membranes

FEBS Letters

Igarashi, Kazuei¹  Kakinuma, Yoshimi¹ 

[1] Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi, Chiba 260, Japan

关键词: Na+-ATPase;    Reconstitution;    Induction;    Streptococcus faecalis;    EDTA ethylenediaminetetraacetic acid;    PMSF;    phenylmethylsulfonyl fluoride;    DCCD;    dicyclohexylcarbodiimide;   

DOI  :  10.1016/0014-5793(90)80382-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Na⁺-stimulated ATPase activity of Streptococcus faecalis was lost by washing the membranes with ethylenediaminetetraacetic acid (EDTA). ATPase activities of both the EDTA extract and the stripped membranes did not show any stimulation by Na⁺ ions. However, the Na⁺-stimulated ATPase was readily reconstituted by an incubation of these fractions combined. It was only reconstituted from the fractions prepared under the condition that the Na⁺-ATPase is amplified, and not from those boiled or digested by trypsin. Thus, the component of Na⁺-ATPase of this organism is capable of being released from the membranes.

【 授权许可】

Unknown