Some features of the Streptococcus faecalis Na+‐ATPase resemble thoseof the vacuolar‐type ATPases

FEBS Letters

Igarashi, Kazuei¹  Kakinuma, Yoshimi¹ 

[1] Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi, Chiba 260, Japan

关键词: Na+-ATPase;    Vacuolar ATPase;    Induction;    Streptococcus faecalis;    EDTA;    ethylenediaminetetraacetic acid;    PAGE;    polyacrylamide gel electrophoresis;    SDS;    sodium dodecyl sulfate;    NEM;    N-ethylmaleimide;   

DOI  :  10.1016/0014-5793(90)80381-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In the ethylenediaminetetraacetic acid (EDTA) extract prepared from the membranes of Streptococcus faecalis, we found the 330-kDa protein that was coordinately increased with the induction of Na⁺-ATPase. It was missed in the EDTA extract of Nakl, a mutant defective in the Na⁺-ATPase, but restored in that of its revertant, NaklR. The 330-kDa protein showed the ATP hydrolytic activity by active staining, and mainly consisted of the polypeptides of 73 kDa, 52 kDa and possibly 38 kDa. In addition, the Na⁺-stimulated ATPase of the membranes was sensitive to both nitrate and N-ethylmaleimide, inhibitors for the vacuolar H⁺-ATPase. Thus, the Na⁺-ATPase of this organism has a structure similar to vacuolar H⁺-ATPase.

【 授权许可】

Unknown