Functional involvement of calcium in the homologous up‐regulation of the 1,25‐dihydroxyvitamin D3 receptor in osteoblast‐like cells

FEBS Letters

Birkenhäger, J.C.¹  Buurman, C.J.¹  van Leeuwen, J.P.T.M.¹  Pols, H.A.P.¹  Schilte, J.P.¹ 

[1] Department of Internal Medicine III, Erasmus University Medical School, Rotterdam, The Netherlands

关键词: 1;    25-Dihydroxyvitamin D3;    Calcium;    1;    25(OH)2D3 receptor up-regulation;   

DOI  :  10.1016/0014-5793(90)81258-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In several cell types 1,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃) causes up-regulation of its receptor. The present study demonstrates that in the osteoblast-like cell line UMR 106 this up-regulation is inhibited by two different calcium channel blockers (nitrendipine, verapamil). Also with chelating extracellular calcium (EGTA) and by inhibition of calcium release from intracellular stores (TMB-8) comparable results were obtained. These findings indicate that calcium is functionally involved in this cellular response to the steroid hormone 1,25(OH)₂D₃. Moreover, data obtained with EGTA show that the 1,25(OH)₂D₃ receptor level is closely regulated by the extracellular calcium concentration.

【 授权许可】

Unknown