Anti‐idiotypic antibody identifies the structural similarity between the phosphatidylcholine‐specific monoclonal antibody and phosphatidylcholine‐specific lipid transfer protein

FEBS Letters

Umeda, Masato¹  Igarashi, Kouji²  Inoue, Keizo¹  Nam, Kyung Soo¹ 

[1] Department of Health Chemistry, Faculty of Pharmaceutical Sciences, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113, Japan;Tosoh Corporation, Tokyo Research Center, 2743-1 Hayakawa, Ayase-shi, Kanagawa-ken 252, Japan

关键词: Lipid-protein interaction;    Monoclonal antibody;    Phosphatidylcholine;    Anti-idiotypic antibody;    Lipid transfer protein;    mAb;    monoclonal antibody;    PC;    phosphatidylcholine;    PS;    phosphatidylserine;    anti-Id;    anti-idiotypic antibody;    ELISA;    enzyme-linked immunosorbent assay;   

DOI  :  10.1016/0014-5793(90)81201-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The polyclonal anti-idiotypic antibody (Anti-Id) has been raised against a monoclonal antibody (mAb) that specifically binds to phosphatidylcholine (PC). The anti-Id bound strongly to PC-specific mAbs, but not to the other mAbs that bind to phosphatidylserine, indicating that the anti-Id recognizes the cross-reactive idiotopes expressed on the PC-specific mAbs. The anti-Id also showed an extensive cross-reaction with the PC-specific lipid transfer protein isolated from bovine liver and inhibited the lipid transfer activity of the protein. These results strongly suggest that the anti-Id recognizes a common structure shared between PC-specific mAbs and the PC-specific lipid transfer protein.

【 授权许可】

Unknown