Production and characterization of monoclonal anti‐idiotypic antibodies to muramylpeptide

FEBS Letters

Mareeva, T.Yu.¹  Golovina, T.N.¹  Kotova, O.V.¹  Nesmeyanov, V.A.¹ 

[1] Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, 117871 Moscow, Russian Federation

关键词: Anti-idiotypic antibody;    Monoclonal antibody;    Muramyl peptide;    MP;    muramyl peptide;    MDP;    MurNAc-Ala-d-iGln;    GMDP;    GlcNAc-β1-4-MurNAc-Ala-d-iGln;    L-GMDP;    GlcNAc-β1-4-MurNAc-Ala-iGln;    GMDP-Lys;    GlcNAc-β1-4-MurNAc-Ala-d-iGln-Lys;    PBS;    0.01 M sodium phosphate buffer;    containing 0.15 M NaCl;    PBST;    PBS containing 0.1% Tween 20;    BSA;    bovine serum albumin;    GMDP-OVA;    GMDP conjugated to ovalbumin;    GMDP-AL;    GMDP conjugated to poly-Lys-poly-Ala polymer;    MeBSA;    methylated bovine serum albumin;   

DOI  :  10.1016/0014-5793(94)01220-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Hybridomas producing monoclonal anti-idiotypic antibodies (anti-id MAbs) to N-acetylglucosaminyl-β1-4-N-acetylmuramyl-alanyl-d-isoglutamine (GMDP) were developed. Three clones of hybridomas demonstrated the properties characteristic for the Ab2β type of anti-id antibody: they bound to Fab-fragments of high-affinity MAb to GMDP; dose-dependent inhibition of this binding by GMDP was observed; immunization of mice with these MAbs resulted in production of GMDP-specific antibodies. When these antibodies were used to stain blots from SDS-PAGE of macrophage lysate, the same receptor proteins were specifically stained as upon staining with ¹²⁵I-labelled GMDP derivative.

【 授权许可】

Unknown