FEBS Letters | |
Cloning and sequencing of a cDNA encoding human milk β‐casein | |
Bergström, Sven1  Sundqvist, Anna Karin3  Hjalmarsson, Karin3  Hemell, Olle2  Andersson, Yvonne2  Lönnerdal, Bo4  | |
[1] Department of Microbiology, University of Umeå, Sweden;Department of Pediatrics, University of Umeå, Sweden;Symbicon AB, Umeå, Sweden;Department of Nutrition, University of California, Davis, CA 95616, USA | |
关键词: Nucleotide sequence; Human milk protein; Calcium-binding site; Sequence comparison; Angiotensin-converting enzyme inhibitor; | |
DOI : 10.1016/0014-5793(90)81142-B | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
A cDNA of 1065 bp encoding the human milk β-casein was cloned and sequenced using a synthetic oligodeoxyribonucleotide probe and a human mammary gland library. The nucleotide (nt) sequence contained an open reading frame sufficient to encode the entire amino-acid (aa) sequence of a β-casein precursor protein consisting of 210 aa and a signal peptide of 15 aa. The nt sequence shows 45–62% homology to those of bovine, ovine, rat, and mouse β-caseins. The highly phosphorylated site, which is responsible for the calcium-binding capacity of β-casein, the signal peptide, and a sequence encoding for an inhibitor to the angiotensin-converting enzyme seem highly conserved among the β-caseins with known sequences.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020293774ZK.pdf | 442KB | download |