Cloning and sequencing of a cDNA encoding human milk β‐casein

FEBS Letters

Bergström, Sven¹  Sundqvist, Anna Karin³  Hjalmarsson, Karin³  Hemell, Olle²  Andersson, Yvonne²  Lönnerdal, Bo⁴ 

[1] Department of Microbiology, University of Umeå, Sweden;Department of Pediatrics, University of Umeå, Sweden;Symbicon AB, Umeå, Sweden;Department of Nutrition, University of California, Davis, CA 95616, USA

关键词: Nucleotide sequence;    Human milk protein;    Calcium-binding site;    Sequence comparison;    Angiotensin-converting enzyme inhibitor;   

DOI  :  10.1016/0014-5793(90)81142-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA of 1065 bp encoding the human milk β-casein was cloned and sequenced using a synthetic oligodeoxyribonucleotide probe and a human mammary gland library. The nucleotide (nt) sequence contained an open reading frame sufficient to encode the entire amino-acid (aa) sequence of a β-casein precursor protein consisting of 210 aa and a signal peptide of 15 aa. The nt sequence shows 45–62% homology to those of bovine, ovine, rat, and mouse β-caseins. The highly phosphorylated site, which is responsible for the calcium-binding capacity of β-casein, the signal peptide, and a sequence encoding for an inhibitor to the angiotensin-converting enzyme seem highly conserved among the β-caseins with known sequences.

【 授权许可】

Unknown