Modulation of calcium mobilization by guanosine 5'‐O‐(2‐thiodiphosphate) in Xenopus oocytes

FEBS Letters

Gillo, Boaz¹  Sealfbn, Stuart C.¹  Mundamattom, Shirly¹ 

[1] Fishberg Center in Neurobiology and Department of Neurology, The Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029, U.S.A.

关键词: Inositol 1;    4;    5-trisphosphate;    Calcium pool;    Calcium channel;    IP3;    inositol 1;    4;    5-trisphosphate;    G-protein;    guanyl nucleotide binding protein;    IP4;    inositol 1;    3;    4;    5-tetrakisphosphate;    GDPßS;    guanosine 5'-O-(2-thiodiphosphate);    ADPßS;    adenosine 5'-O-(2-thiodiphosphate);    EGTA;    ethylene glycol-bis(ß-aminoethyl ether) N;    N;    N';    N'-tettaacetic acid;   

DOI  :  10.1016/0014-5793(90)81138-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the effect of intracellular loading of the non-hydrolyzable guanosine 5'-diphosphate analogue GDPßS on calcium mobilization by IP₃ and on calcium influx in Xenopus oocytes. Assayed by two electrode voltage-clamp recording, GDPßS-loaded oocytes demonstrated a marked augmentation of the fast component of the response to IP₃ injection, an attenuation of the slow component and an increase in membrane calcium permeability. These effects on calcium mobilization suggest that gdpβs may facilitate calcium translocation both across the plasma membrane and between different intracellular calcium pools.

【 授权许可】

Unknown