The effect of adenosine on the fluorescence responses of chlorotetracycline‐loaded human polymorphonuclear leukocytes

FEBS Letters

Ito, Setsuko¹  Tsuruta, Satoru²  Mikawa, Haruki² 

[1] Ijinkai Takeda Hospital, Kyoto, Japan;Department of Pediatrics, Kyoto University Hospital, 56 Shogoinkawaharamachi, Sakyo-ku, Kyoto-city, 606 Japan

关键词: Adenosine;    Chlorotetracycline;    FMLP;    Adenosine A2 receptor;    Membrane-bound calcium;   

DOI  :  10.1016/0014-5793(90)81018-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Chlorotetracycline has been used in human polymorphonuclear leukocytes as a probe to investigate the state of membrane-bound calcium. We examined the effect of adenosine on the fluorescence responses of CTC-loaded PMNs stimulated with the synthetic chemotactic peptide, formylmethionyl-leucyl-phenylalanine. Adenosine inhibited the decrease in CTC fluorescence in a dose-dependent fashion and its effect was reversed by theophylline, an adenosine receptor antagonist. Removal of extracellular adenosine by incubating PMNs with adenosine deaminase abolished the effect of adenosine. These data suggest that adenosine inhibits the release of membrane-bound calcium in PMNs that normally occurs in response to chemotactic stimuli, acting via PMN surface adenosine receptors.

【 授权许可】

Unknown