Localization of aldolase C mRNA in brain cells

FEBS Letters

Vibert, Magdeleine²  Berwald-Netter, Yoheved¹  Skala, Henriette²  Kahn, Axel²  Popovici, Théodora² 

[1] Laboratoire de Biochimie Cellulaire, Collége de France, 11 place Marcelin Berthelot, 75005 Paris, France;INSERM U 129, 24 rue du Fg St Jacques, 75014 Paris, France

关键词: Aldolase C;    Brain cell;    Hybridization in situ;   

DOI  :  10.1016/0014-5793(90)81005-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The expression of aldolase C and aldolase A mRNA was assessed by Northern blot hybridization using RNAs purified from cultured rat and mouse brain neurons and astroglial cells. Neurons were found to contain about 4-fold more aldolase C mRNA and about twice as much aldolase A mRNA than astroglia. Analysis of the cellular localization of aldolase C mRNA by in situ hybridization to brain slices showed a predominantly neuronal labeling with an irregular distribution. A strong signal was observed in Purkinje cell somata and a weaker signal in subpopulations of neurons in cerebral cortex, striatum, hippocampus, hypothalamic nuclei and primary olfactory cortex.

【 授权许可】

Unknown