Detection of tryptase TL2 and CD26 antigen in brain‐derived cells non‐permissive to T ‐cell line‐tropic human immunodeficiency virus type 1

FEBS Letters

Shimizu, Nobuaki²  Kido, Hiroshi¹  Hoshino, Hiroo²  Kobayashi, Mitsuru²  Liu, Hui-Yu² 

[1] Division of Enzyme Chemistry, Institute for Enzyme Research, The University of Tokushima, Tokushima 770, Japan;Department of Hygiene and Virology, Gunma University School of Medicine, Showa-machi, Maebashi, Gunma 371, Japan

关键词: HIV-1;    Tryptase TL2;    CD26;    Brain cell;    Tropism;   

DOI  :  10.1016/0014-5793(94)01394-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tryptase TL₂ purified from MOLT-4 human T cells binds to the envelope protein of human immunodeficiency virus type 1 (HIV 1). Tryptase TL₂ and CD26 antigen are supposed to play roles in HIV-1 entry into cells. Although CD4 is a principal receptor for HIV-1, brain cells expressing the CD4 antigen are not permissive to HIV-1 strains infectious to monocyte or T-cell lines. We examined whether the non-permissiveness of the brain-derived cells to standard HIV-1 strains could be explained by a lack of tryptase TL₂ or CD26. Western blots showed that the amounts of tryptase TL2 expressed in cell lysates prepared from the brain-derived cells were similar to those prepared from various cells susceptible to HIV-1 strains. Furthermore, flow cytometry revealed the presence of the CD26 antigen on the cell surface of many types of cells. The resistance of the brain-derived cells to standard HIV-1 strains is not due to a lack of tryptase TL₂ or CD26.

【 授权许可】

Unknown