Cis‐acting elements in the promoter region of the human aldolase C gene

FEBS Letters

Buono, Pasqualina¹  Izzo, Paola¹  de Conciliis, Lisa¹  Salvatore, Francesco¹  Olivetta, Eleonora¹ 

[1] Dipartimento di Biochimica e Biotecnologie Mediche, Facoltà di Medicina e Chirurgia, Università di Napoli ‘Federico II’, Via S. Pansini 5, 1-80131, Napoli, Italy

关键词: Aldolase C;    Gene expression;    Neuroblastoma cell (SKNBE);    cis-acting elements;    Sp1 transacting factor;    CAT;    chloramphenicol acetyl transferase;    DMEM;    Dulbeeco's modification of Eagle's medium;    MMLV;    murine mammary leukemia virus. Enzymes: fructose-1;    6-bisphosphate aldolase (EC 4.1.2.13);   

DOI  :  10.1016/0014-5793(93)80936-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We investigated the cis-acting sequences involved in the expression of the human aldolase C gene by transient transfections into human neuroblastoma cells (SKNBE). We demonstrate that 420 bp of the 5'-flanking DNA direct at high efficiency the transcription of the CAT reporter gene. A deletion between − 420 bp and − 164 bp causes a 60% decrease of CAT activity. Gel shift and DNase I footprinting analyses revealed four protected elements: A, B, C and D. Competition analyses indicate that Spl or factors sharing a similar sequence specificity bind to elements A and B, but not to elements C and D. Sequence analysis shows a half palindromic ERE motif (GGTCA), in elements B and D. Region D binds a transactivating factor which appears also essential to stabilize the initiation complex.

【 授权许可】

Unknown