Modelling heme d 1

FEBS Letters

Andersson, Laura A.²  Chang, Chi K.³  Wu, Weishih³  Timkovich, Russell¹  Loehr, Thomas M.² 

[1] Department of Chemistry, University of Alabama, University, AL 35486, USA;Department of Chemical and Biological Sciences, Oregon Graduate Institute, Beaverton, OR 97006-1999, USA;Department of Chemistry, Michigan State University, East Lansing, MI 48824 USA

关键词: Nitrite reductase;    Pseudomonas aeruginosa;    Heme d 1;    Resonance Raman;    Porphyrin;    Saturated porphyrin;    Hydroporphyrin;    iBC;    isobacteriochlorin;    RR;    resonance Raman;    FTIR;    Fourier transform infrared;    TME;    tetramethylester;    Cu-d 1;    the copper(II) TME of P. aeruginosa heme d 1;    THP;    tetrahydroporphyrin;   

DOI  :  10.1016/0014-5793(90)80946-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The heme d ₁ macrocycle of Ps. aeruginosa dissimilatory nitrite reductase is an iron porphyrin-3,8-dione with a 17-acrylate substituent. We have compared the RR properties of Cu-d ₁, the copper(II) TME of extracted heme d ₁, with those of models that differ only with respect to the acrylate: Cu-17-acrylate-mesoporphyrin-3,8-dione (2) and Cu-mesoporphyrin-3,8-dione (3). The RR spectrum of Cu-d ₁ is very similar to that of 2, including v(C=O) at ~ 1720 cm⁻¹. Replacement of the acrylate with propionate changes the spectrum markedly. For example, the v(C=O) mode of 3 shifts to 1712 cm⁻¹, and peaks of Cu-d ₁ and 2 at ~ 1400 and ~ 1535 cm⁻¹ are shifted or absent from the spectrum of 3. FTIR spectra of 2 and 3 also differ in their ν_oxo(C=O) frequencies. The acrylate thus has a surprisingly strong influence on the electronic structural and spectral properties of heme d ₁. These data provide a foundation for studies of the novel biological porphyrindione macrocycles.

【 授权许可】

Unknown