The human islet amyloid polypeptide (IAPP) gene

FEBS Letters

Rorsman, Fredrik¹  Stenman, Göran²  Betsholtz, Christer¹  Christmanson, Lars¹  Westermark, Per³ 

[1] Department of Pathology, University Hospital, S-751 85 Uppsala, Sweden;Department of Oral Pathology, Gothenburg University, Box 33070, S-400 33 Göteborg, Sweden;Department of Pathology, University Hospital. S-581 85 Linköping, Sweden

关键词: hIAPP;    human islet amyloid polypeptide;    cDNA;    complementary deoxyribonucleic acid;    mRNA;    messenger ribonucleic acid;    bp;    base pairs;    kbp;    kilobase pairs;    CAT;    chloramphenicol acetyltransferase;   

DOI  :  10.1016/0014-5793(90)80314-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We report the isolation and characterization of the human gene encoding islet amyloid polypeptide (IAPP). Previously characterized cDNA sequences correspond to three exons of which the first is noncoding. A functional promoter region was identified in the 5' flanking DNA; however, this was farther upstream than expected. Northern blot analysis of human insulinoma RNA revealed three IAPP mRNAs of sizes 1.2, 1.8 and 2.1 kb, in agreement with three polyadenylation signals present in the 3' end of the gene. In situ hybridization to metaphase chromosomes resulted in two distinct peaks on chromosome 12, at 12p12-p13 and 12q13-q14. Southern blot analysis of genomic DNA suggested a single IAPP locus but also indicated the presence of additional homologous sequences in human genomic DNA.

【 授权许可】

Unknown