Molecular cloning, functional expression and localization of a novel inward rectifier potassium channel in the rat brain

FEBS Letters

Zanelli, Jill S.²  Fass, David N.¹  Kurachi, Yoshihisa²  Takahashi, Naohiko²  Koyama, Hidekazu²  Morishige, Ken-Ichirou² 

[1] Departments of Hematology , Mayo Clinic, Rochester, MN 55905, USA;Division of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA

关键词: Inward rectifier potassium channel;    cDNA library;    Rat brain;    Northern blot analysis;    Xenopus oocyte;    IRK;    inward rectifier potassium channel;    cDNA;    complementary deoxyribonucleic acid;    cRNA;    complementary ribonucleic acid;    mRNA;    messenger ribonucleic acid;    SSC;    saline-sodium citrate;    SDS;    sodium dodecyl sulfate;    GAPDH;    glyceraldehyde-3-phosphate-dehydrogenase;   

DOI  :  10.1016/0014-5793(94)80478-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned a novel inward rectifier potassium channel from a rat brain cDNA library and designated it RB-IRK2. The rat brain cDNA library was screened using a fragment of the mouse macrophage IRK1 cDNA as a probe. The amino acid sequence of RB-IRK2 shares 70%, 40% and 45% identity to mouse IRK1, rat ROMK1 and rat GIRK1, respectively. Xenopus oocytes injected with cRNA derived from RB-IRK2 expressed a potassium current which showed inward-rectifying channel characteristics similar to the IRK1 current, but distinct from the ROMK1 or the GIRK1 currents. However, the localization of RB-IRK2 mRNA in rat tissues, assessed by the Northern blot analysis, differed from that of mouse IRK1. These results indicate that the IRK family is composed of multiple genes, which express in different tissues and therefore may play heterogenous functional roles in various organs, including rat central nervous system.

【 授权许可】

Unknown