Molecular cloning, functional expression and localization of an inward rectifier potassium channel in the mouse brain

FEBS Letters

Kurach, Yoshihisa³  Zanelli, Jill S.³  Peterson, Christine⁴  Jenkins, Nancy A.²  Mori, Nozomu⁴  Takahashi, Naohiko³  Copeland, Neal G.²  Findlay, Ian¹  Koyama, Hidekazu³  Morishige, Ken-Ichirou³ 

[1] Universite de Tour, Tour, France;Mammalian Genetics Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick, MD 21702-1201, USA;Division of Cardiovascular Diseases, Department of Internal Medicine, Mayo Clinic, Rochester, MN 55905, USA;Ethel Percy Andrus Gerontology Center, University of Southern California, Los Angeles, CA 90089, USA

关键词: Inward rectifier potassium channel;    cDNA library;    Mouse brain;    Xenopus oocyte;    Hybridization;    in situ;    Chromosome mapping;    IRK;    inward rectifier potassium channel;    cDNA complementary deoxyribonucleotic arid;    cRNA;    complementary ribonucleic arid;    mRNA;    messenger ribonucleic arid;    SCC;    saline-sodium ritrate;    SDS;    sodium dodecyl sulfate;    TH;    tyrosine hydroxylase;    GABA;    γ-amino-n-butyric acid;   

DOI  :  10.1016/0014-5793(93)80840-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have cloned an inward-rectifier potassium channel from a mouse brain cDNA library, studied its distribution in the brain by in situ hybridization and determined the chromosomal localization of the gene. A mouse brain cDNA library was screened using a fragment of the mouse macrophage IRK1 cDNA as a probe. Two duplicate clones of ~5.5 kb were obtained. Xenopus ococytes injected with cRNA derived from the clone expressed a potassium channel with inwardly rectifying channel characteristics. The amino acid sequence of the clone was identical to that of IRK1 recently cloned from a mouse macrophage cell line. In situ hybridization study showed the mouse brain IRK1 to be generally distributed throughout the brain, but in particular subsets of neurons at high levels. The gene was placed in the distal region of mouse chromosome 11, which contains several uncloned neurological mutations. These results provide the first demonstration of the cloning and distribution of an inward rectifier potassium channel from the nervous system.

【 授权许可】

Unknown