期刊论文详细信息
FEBS Letters | |
The role of a conserved guanosine residue in the hammerhead‐type RNA enzyme | |
Uesugi, Seiichi1  Sakata, Takeshi1  Hiroaki, Hidekazu1  Odai, Osamu1  Tanaka, Toshiki2  | |
[1] Faculty of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565, Japan;Protein Engineering Research Institute, Suita, Osaka 565, Japan | |
关键词: Synthetic oligoribonucleotide; RNA enzyme; Hammerhead structure: NMR; Conformation; | |
DOI : 10.1016/0014-5793(90)80311-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
We have designed a hammerhead-type RNA system which consists of three RNA fagments for normal and modified complexes which contain a non-cleavable substrate with 2'-O-methylcytidine and a guanosine-to-inosine replaced enzyme. Examination of the RNA-cleaving activity and conformational properties of the complexes suggests that the 2-amino group of a conserved guanosine residue in the loop region plays an important role for maintaining both the activity and loop conformation.
【 授权许可】
Unknown
【 预 览 】
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RO201912020293570ZK.pdf | 288KB | download |