The role of a conserved guanosine residue in the hammerhead‐type RNA enzyme

FEBS Letters

Uesugi, Seiichi¹  Sakata, Takeshi¹  Hiroaki, Hidekazu¹  Odai, Osamu¹  Tanaka, Toshiki² 

[1] Faculty of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565, Japan;Protein Engineering Research Institute, Suita, Osaka 565, Japan

关键词: Synthetic oligoribonucleotide;    RNA enzyme;    Hammerhead structure: NMR;    Conformation;   

DOI  :  10.1016/0014-5793(90)80311-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have designed a hammerhead-type RNA system which consists of three RNA fagments for normal and modified complexes which contain a non-cleavable substrate with 2'-O-methylcytidine and a guanosine-to-inosine replaced enzyme. Examination of the RNA-cleaving activity and conformational properties of the complexes suggests that the 2-amino group of a conserved guanosine residue in the loop region plays an important role for maintaining both the activity and loop conformation.

【 授权许可】

Unknown