期刊论文详细信息
FEBS Letters | |
Construction of a series of several self‐cleaving RNA duplexes using synthetic 21‐mers | |
Iwai, Shigenori1  Ohtsuka, Eiko1  Koizumi, Makoto1  | |
[1] Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan | |
关键词: Synthetic oligoribonucleotide; RNA self-cleavage; Satellite RNA; Hammerhead self-cleaving RNA; (Newt); | |
DOI : 10.1016/0014-5793(88)80004-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Two fragments (21-mers) containing consensus sequences for the self-cleavage domain in transcripts of satellite DNA of the newt were chemically synthesized and found to be cleaved in the presence of Mg2+. The cleaved product contained the 3′-terminal 2′,3′-cyclic phosphate. Twenty-five combinations of partially double-stranded 21-mer RNA which contained different bases within the consensus sequences and at the cleavage sites were tested for self-cleavage. It seemed that guanosine 3′-phosphate was not susceptible to transesterification at the cleavage site.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020290245ZK.pdf | 297KB | download |