Construction of a series of several self‐cleaving RNA duplexes using synthetic 21‐mers

FEBS Letters

Iwai, Shigenori¹  Ohtsuka, Eiko¹  Koizumi, Makoto¹ 

[1] Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan

关键词: Synthetic oligoribonucleotide;    RNA self-cleavage;    Satellite RNA;    Hammerhead self-cleaving RNA;    (Newt);   

DOI  :  10.1016/0014-5793(88)80004-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two fragments (21-mers) containing consensus sequences for the self-cleavage domain in transcripts of satellite DNA of the newt were chemically synthesized and found to be cleaved in the presence of Mg²⁺. The cleaved product contained the 3′-terminal 2′,3′-cyclic phosphate. Twenty-five combinations of partially double-stranded 21-mer RNA which contained different bases within the consensus sequences and at the cleavage sites were tested for self-cleavage. It seemed that guanosine 3′-phosphate was not susceptible to transesterification at the cleavage site.

【 授权许可】

Unknown