Cleavage of specific sites of RNA by designed ribozymes

FEBS Letters

Iwai, Shigenori¹  Ohtsuka, Eiko¹  Koizumi, Makoto¹ 

[1] Faculty of Pharmaceutical Sciences, Hokkaido University, Sapporo 060, Japan

关键词: Synthetic oligoribonucleotide;    RNA enzyme;    Endonucleolytic activity;    RNA sequence recognition;   

DOI  :  10.1016/0014-5793(88)80935-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two ribozymes were designed for site-specific cleavage of RNA. A UA site in an undecaribonucleotide was cleaved by a ribozyme consisting of two partially paired oligoribonucleotides with chain lengths of 19 and 15. The other ribozyme, which consists of a 19-mer and a 13-mer, recognized a UC sequence at positions 42 and 43 of 5 S rRNA.

【 授权许可】

Unknown