Microheterogeneity of parsley plastocyanin

FEBS Letters

Egorov, Tsezi A.¹  Dimitrov, Mitko I.²  Donchev, Anthony A.² 

[1] Institute of Molecular Biology V.A. Engelhardt, Academy of Sciences of the USSR, Moscow B-334, USSR;Central Laboratory of Biophysics, Bulgarian Academy of Sciences, 1040 Sofia, Bulgaria

关键词: Primary structure;    Plastocyanin a;    Plastocyanin b;    Microheterogeneity;    Parsley;    T;    tryptic peptides of PCa and PCb;    Sa and Sb;    Staphylococcal protease peptides of PCa and PCb;    respectively;   

DOI  :  10.1016/0014-5793(90)80904-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A procedure for isolation of two iso-plastocyanins from parsley has been described here. Three consecutive Chromatographic steps on DE-52-Whatman cellulose were applied for isolation of two total plastocyanin (PC) fractions, oxidized [PC(II)] and reduced [PC(I)]. By chromatofocusing of PC(II) on Polybuffer exchanger 74 two different plastocyanins, designated as plastocyanin a (PCa) and plastocyanin b (PCb), were obtained. The isoelectric points (pI) of PCa and PCb at 10°C are 4.16 and 4.14, respectively. The complete amino acid sequences of PCa and PCb were determined. The two iso-proteins consist of 97 amino acid residues and differ only at sequence position 53, where Glu in PCa is replaced by Asp in PCb.

【 授权许可】

Unknown