FEBS Letters | |
The amino‐terminal sequences of four major carp γ‐crystallin polypeptides and their homology with frog and calf γ‐crystallins | |
Chen, Shun-Wen1  Chiou, Shyh-Horng1  Lo, Tung-Bin1  | |
[1] Institute of Biochemical Sciences, National Taiwan University and Institute of Biological Chemistry, Academia Sinica, PO Box 23-106, Taipei, Taiwan, Republic of China | |
关键词: Lens crystallin Protein sequence Sequence homology Microheterogeneity Multigene family; | |
DOI : 10.1016/0014-5793(86)81093-6 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Four major γ-crystallin subfractions have been isolated from the carp (Cyprinus carpio) and their N-terminal sequences determined by Edman protein sequencing. Extensive homologies indicative of close relatedness in their primary structure were found in these four γ-crystallin polypeptides. Comparison of the carp N-terminal sequences with those of mammalian and amphibian γ-crystallins also showed a high degree of homology present in their N-terminal segments despite the dissimilarity of amino acid compositions of fish γ-crystallins to those of higher classes of vertebrates. The distinct yet closely-related partial sequences of carp γ-crystallins could account for the profound microheterogeneity detected in the characterization of carp crystallins, suggesting the presence of a multigene family for γ-crystallin in the lowest class of vertebrates, i.e. the fish.
【 授权许可】
Unknown
【 预 览 】
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