Comparison of the Dl/D2/cytochrome b559 reaction centre complex of photosystem two isolated by two different methods

FEBS Letters

Chapman, D.J.³  Booth, P.²  Porter, G.²  Crystall, B.²  Gounaris, K.¹  Klug, D.R.²  Barber, J.³  Giorgi, L.B.² 

[1] Biochemistry Department, Biology Department, Imperial College, London SW7, UK;Photochemistry Research Group, Biology Department, Imperial College, London SW7, UK;AFRC Photosynthesis Research Group, Biology Department, Imperial College, London SW7, UK

关键词: Photosystem 2;    Reaction centre;    Pigment;    Cytochrome b559;    Time-resolved chlorophyll fluorescence measurement;    PS2;    photosystem 2;    RC-1;    reaction centre prepared as in [14;    15];    RC-2;    reaction centre prepared as in [9];    Cyt b559;    cytochrome b559;    Chl a;    chlorophyll a;    Pheo a;    pheophytin a;    CP47;    47 kDa chlorophyll binding protein encoded by the psbB gene;    D1 polypeptide;    product of the psbA gene;    D2 polypeptide;    product of the psbD gene;   

DOI  :  10.1016/0014-5793(90)80890-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Photosystem 2 reaction centre complexes prepared either by solubilisation with Triton X-100 and subsequent exchange into dodecyl maltoside or by a procedure involving a combination of dodecyl maltoside and LiC10₄, were characterised in terms of chlorophyll a, pheophytin a, β-carotene and cytochrome b559 content. Time-resolved chlorophyll fluorescence decay kinetics were measured using both types of complexes. Our data show that the isolated photosystem two reaction centre complex contain, for two pheophytin a molecules, close to six chlorophyll a, two β-carotene and one cytochrome b559. No major differences were observed in the composition or the kinetic characteristics measured in the samples prepared by the different procedures. Time-resolved fluorescence measurements indicate that more than 94% of the chlorophyll a in both preparations is coupled to the reaction centre complex.

【 授权许可】

Unknown