Site‐specific mutagenesis of the reaction centre from Rhodobacter sphaeroides studied by Fourier transform Raman spectroscopy: mutations at tyrosine M210 do not affect the electronic structure of the primary donor

FEBS Letters

Hunter, C.Neil¹  Mattioli, Tony A.²  Jones, Michael R.¹  Robert, Bruno²  Heer-Dawson, Manuel² 

[1] Robert Hill Institute for Photosynthesis and Krebs Institute for Biomolecular Research, Department of Molecular Biology and Biotechnology, University of Sheffield, Western Bank, Sheffield, S10 2UH, UK;Section de Biophysique des Protéines et des Membranes, DBCM/CEA and URA CNRS 1290, C.E. Saclay, 91191 GiflYvette Cedex, France

关键词: Reaction centre;    Site-directed mutagenesis;    FT-Raman;    Tyrosine M210;    Electron transfer;    Rhodobacter sphaeroides;    Rb.;    Rhodobacter;    RC;    reaction centre;    P;    primary donor;    P+.;    primary donor radical cation;    P∗;    primary donor first excited singlet state;    Bchi;    bacteriochlorophyll;    Bphe;    bacteriopheophytin;    HL;    active branch acceptor Bphe;    HM;    inactive branch Bphe;    BL.;    active branch accessory Bchl;    BM;    inactive branch accessory Bchl;    PM and PL;    Bchls of the primary donor;    QA;    primary acceptor quinone;    QB;    secondary acceptor quinone;    FT;    Fourier transform;    ENDOR;    electron nuclear double resonance;    LDAO;    N;    N-dimethyldodecylamine-N-oxide;   

DOI  :  10.1016/0014-5793(94)80376-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of mutation of residue tyrosine M210 on the primary donor bacteriochlorophylls have been investigated by near infrared FT-Raman spectroscopy in reaction centres purified from an antenna-deficient strain of Rhodobacter sphaeroides. We find that mutation at the M210 position does not significantly perturb the distribution of the unpaired electron over the pair of bacteriochlorophyll molecules which constitute the primary donor radical cation. We conclude, therefore, that the effects of mutation of tyrosine M210 on the rate and asymmetry of primary electron transfer in reaction centres cannot be ascribed to a change in the electronic structure of the primary donor.

【 授权许可】

Unknown