Human c‐myc gene contains a regulatory site similar to consensus of interferon response sequence (IRS)

FEBS Letters

Alexandrova, N.M.¹  Chernov, B.K.¹  Ulyanov, N.B.¹  Itkes, A.V.¹  Kisselev, L.L.¹  Imamova, L.R.¹  Tulchinsky, E.M.¹ 

[1] Engelhardt Institute of Molecular Biology, USSR Academy of Sciences. Moscow 117984, USSR

关键词: c-myc gene;    Interferon-dependent gene;    Transcription;   

DOI  :  10.1016/0014-5793(90)80885-M

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of c-myc proto oncogene is regulated by multiple mechanisms. Here, we report that the consensus of the regulatory region of interferondependent genes, GGAAAN₁₋₃ GAAA, was found after computer search in the 5'-terminal flank of human c-myc gene in position (−76:−67). In vitro transcription of c-myc gene fragments showed that the consensus region competes with oligonucleotide GGGAAAATGAAACT for binding to specific protein(s). This oligonucleotide was shown to bind selectively the interferon-dependent positive transcription factor [1]. Transcription of c-myc fragments lacking 5'-terminal region up to positions −101 or +71 was initiated at two sites located in the first intron. These sites did not coincide with P₁ in vivo RNA cap-site. Binding of the protein factor(s) to the regulatory region of c-myc gene −76:−67 blocked the in vitro transcription initiated in the first intron.

【 授权许可】

Unknown