FEBS Letters | |
Human c‐myc gene contains a regulatory site similar to consensus of interferon response sequence (IRS) | |
Alexandrova, N.M.1  Chernov, B.K.1  Ulyanov, N.B.1  Itkes, A.V.1  Kisselev, L.L.1  Imamova, L.R.1  Tulchinsky, E.M.1  | |
[1] Engelhardt Institute of Molecular Biology, USSR Academy of Sciences. Moscow 117984, USSR | |
关键词: c-myc gene; Interferon-dependent gene; Transcription; | |
DOI : 10.1016/0014-5793(90)80885-M | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Expression of c-myc proto oncogene is regulated by multiple mechanisms. Here, we report that the consensus of the regulatory region of interferondependent genes, GGAAAN1−3 GAAA, was found after computer search in the 5'-terminal flank of human c-myc gene in position (−76:−67). In vitro transcription of c-myc gene fragments showed that the consensus region competes with oligonucleotide GGGAAAATGAAACT for binding to specific protein(s). This oligonucleotide was shown to bind selectively the interferon-dependent positive transcription factor [1]. Transcription of c-myc fragments lacking 5'-terminal region up to positions −101 or +71 was initiated at two sites located in the first intron. These sites did not coincide with P1 in vivo RNA cap-site. Binding of the protein factor(s) to the regulatory region of c-myc gene −76:−67 blocked the in vitro transcription initiated in the first intron.
【 授权许可】
Unknown
【 预 览 】
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