Cyclic AMP and c‐myc gene expression in PY815 mouse mastocytoma cells

FEBS Letters

De Feyter, R.¹  Ralph, R.K.¹  Le Gros, J.¹ 

[1] Department of Cell Biology, University of Auckland, Auckland, New Zealand

关键词: Cyclic AMP;    c-myc gene;    Gene expression;    PY815 mouse mastocytoma cell;   

DOI  :  10.1016/0014-5793(85)81329-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possibility was examined that inhibition of growth of PY815 mouse mastocytoma cells by N ⁶,O ^2'-dibutyryladenosine 3',5'-cyclic monophosphate (DB cyclic AMP) results from inhibition of c-myc gene expression. Temporary increases in c-myc RNA which occurred soon after DB cyclic AMP treatment and upon removal of the drug were not consistent with direct inhibition of c-myc gene expression by DB cyclic AMP. The increases in c-myc RNA coincided with the passage through, or accumulation of cells in late G₁ - early S phase. It is proposed that cyclic AMP may stimulate c-myc gene expression which normally occurs only in late G₁ - early S phase in PY815 cells and that cyclic AMP prevents c-myc expression in cells at other phases of the cell cycle by inhibiting their progression past a cyclic AMP-sensitive restriction point in early G₁ phase.

【 授权许可】

Unknown