McrI: a novel class‐II restriction endonuclease from Micrococcus cryophilus recognizing 5'‐CGRY/CG‐3'

FEBS Letters

Reischl, Udo¹  Kessler, Christoph¹  Brensing-Küppers, Jutta¹  Jarsch, Michael¹  Kaluza, Klaus¹  Schmitz, Gudrun S.¹ 

[1] Boehringer Mannheim GmbH, Biochemical Research Center, Department of Genetics, Nonnenwald 2, D-8122 Penzberg, FRG

关键词: Restriction endonuclease;    Hexanucleotide recognition sequence;    3'-protruding terminus;    CpG-dinucleotide;    HTF-island;   

DOI  :  10.1016/0014-5793(90)80252-E

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new class-II restriction endonuclease, McrI, with a novel sequence specificity as isolated from the Gram-positive eubacterium Micrococcus cryophilus. McrI recognizes the palindromic hexanucleotide sequence The novel enzyme in the presence of Mg²⁺-ions cleaves specifically both strands as indicated by the arrows. The staggered cuts generate 3'-protruding ends with single-stranded 5'-RY-3' dinucleotide extensions. The McrI recognition sequence was deduced from mapping data on DNAs of bacteriophages φX174RF and M13mp18RF characterized by one and four cleavage sites, respectively. The cut positions within both strands of the recognition sequence were determined in sequencing experiments by analyzing hydrolysis of phosphodiester bonds within a Polylinker region of M13mp18RF DNA containing an additional McrI recognition site including treatment with T4 DNA polymerase. The novel enzyme may be a useful tool for cloning experiments by completion of the enzymes EclXI (5'-C/GGCCG-3'), NotI (5'-GC/GGCCGC-3'), PvuI (5'-CGAT/CG-3') as well as EaeI (5'-Y/GGCCR-3') and XhoII (5'-Y/GATCR-3') characterized by partly identical sequence specificities.

【 授权许可】

Unknown