AsnI: A novel class II restriction endonuclease from Arthrobacter sp., strain N‐CM, recognizing 5′‐AT/TAAT‐3′

FEBS Letters

Berger, Gerhard¹  Sagmeister, Christine¹  Kessler, Christoph¹  Rexer, Bernhard U.¹  Glück, Barbara¹  Jarsch, Michael¹ 

[1] Boehringer Mannheim GmbH, Biochemical Research Center, Department of Molecular Biology, Nonnenwald 2, D-8133-Penzberg, FRG

关键词: Restriction endonuclease;    Hexanucleotide recognition sequence;    T/A-nucleotide;    5′-Protruding terminus;    Klenow enzyme;    T4 polynucleotide kinase;    T4 DNA polymerase;    T4DNA ligase;   

DOI  :  10.1016/0014-5793(88)81271-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new class II restriction endonuclease, AsnI, with a novel sequence specificity was isolated from the Gram-positive eubacterium Arthrobacter species, strain N-CM. AsnI recognizes the unambiguously defined palindromic hexanucleotide consisting of A- and T-residues. The novel enzyme in the presence of Mg²⁺ cleaves specifically both strands as indicated by the arrows. The staggered cuts generate 5′-protruding ends with single-stranded 5′-TA-3′ dinucleotide extensions. The novel enzyme may be a useful tool for cloning experiments by complementation of the few enzymes such as PstI and PvuI cutting only once in the Amp^r-gene of plasmids pBR322 and pBR328.

【 授权许可】

Unknown