Methylation of repetitive DNA sequences in the brain during aging of the rat

FEBS Letters

Kanungo, M.S.¹  Rath, P.C.¹ 

[1] Molecular Biology Laboratory, Department of Zoology, Banaras Hindu University, Varanasi-221 005, India

关键词: DNA methylation;    Restriction endonuclease;    Repetitive DNA;    Age-related change;   

DOI  :  10.1016/0014-5793(89)81191-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Methylation of repetitive DNA sequences (RDS) of the genomic DNA of the brain of 15- and 88-week old rats was analysed by digestion with HpaII, MspI, EcoRI + HpaII and EcoRI + MspI followed by end-labelling. doublets are present in two RDS of ∼ 5 and 0.4 kb, and are also randomly distributed throughout the genome. Hemimethylated doublets also occur. Both and doublets are found more in the old than in the young. This age-related increase in DNA methylation occurs both at CCGG sites of the RDS and in the entire genome. Such increase in DNA methylation may alter chromatin conformation and gene expression in the brain as the rat ages.

【 授权许可】

Unknown