Identification of the binding sites of benzyl penicilloyl, the allergenic metabolite of penicillin, on the serum albumin molecule

FEBS Letters

Yvon, Mireille¹  Anglade, Patricia¹  Wal, Jean-Michel¹ 

[1] Laboratoire des Sciences de la Consommation INRA-CRJ, 78350 Jouy-en-Josas, France

关键词: Albumin;    Penicillin;    Benzyl penicilloyl;    Lysine residue;    Serine residue;   

DOI  :  10.1016/0014-5793(90)81382-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tryptic digests of fragment A_{299 585} of penicilloylated serum albumin obtained from two penicillin-treated patients or prepared by in vitro conjugation, were analyzed by a tandem immunoaffinity reversed-phase HLPC. Determinations of benzyl penicilloyl groups (BPO) were performed on the different fractions. Three BPO containing peptides were identified by their amino acid sequence and the bound BPO were located on lysines 432, 541 and 545. Six major BPO binding sites were thus identified on the whole albumin molecule. All of them are lysine residues and correspond to a limited number of definite structures in which lysine and serine residues appear to be closely associated.

【 授权许可】

Unknown