Binding of benzyl penicilloyl to human serum albumin Evidence for a highly reactive region at the junction of domains 1 and 2 of the albumin molecule

FEBS Letters

Yvon, Mireille¹  Anglade, Patricia²  Wal, Jean-Michel¹ 

[1] Laboratoire des Sciences de la Consommation, INRA-CRJ, 78350 Jouy-en-Josas, France;Station de Recherches Laitières, INRA-CRJ, 78350 Jouy-en-Josas, France

关键词: Albumin;    Penicillin;    Benzyl penicilloyl;    Lysine;    Serine;   

DOI  :  10.1016/0014-5793(89)81351-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Tryptic digests of fragment C_124–298 of penicilloylated serum albumin, obtained from a penicillin-treated patient or prepared by in vitro conjugation, were analyzed by HPLC. Determinations of benzyl penicilloyl groups (BPO) were performed on the different fractions. Three BPO-containing peptides were identified by their amino acid sequence and the bound BPO was located on lysines 190, 195 and 199 and serine 193. These four main BPO-binding sites are all located on a very short region (10 amino acid residues) of the albumin molecule at the junction of domains 1 and 2.

【 授权许可】

Unknown