Receptor‐active glycolipids of epithelial cells of the small intestine of young and adult pigs in relation to susceptibility to infection with Escherichia coli K99

FEBS Letters

Karlsson, Karl-Anders¹  Willemsen, Peter²  Teneberg, Susann¹  de Graaf, Frits K.² 

[1] Department of Medical Biochemistry, University of Göteborg, PO Box 33031, S-400 33 Göteborg, Sweden;Biological Laboratory, Department of Microbiology, Vrije Universiteit, 1081 HV Amsterdam, The Netherlands

关键词: Bacterial adhesion: E. coli K99;    Pig intestine;    Receptor;    Glycolipid;    Sialyllactosylceramide;    N-Glycolylneuraminic acid;   

DOI  :  10.1016/0014-5793(90)80693-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Glycolipids from mucosa scrapings of small intestine of neonatal and adult pigs were tested by the thin-layer chromatogram overlay assay for the binding of Escherichia coli K99. There was practically no binding to acid or non-acid glycolipids of adult pig, known to be resistant to infection with this bacterium. However, piglets, which are susceptible to infection, showed a clear binding to a doublet band in the acid glycolipid fraction. The receptor-active glycolipid was isolated and shown by mass spectrometry, NMR spectroscopy and degradation methods to be NeuGcα-3Galß4GlcßCer (NeuGc-GM3), the two bands being due to heterogeneity of the ceramide. When tested against various reference glycolipids, NeuAc-GM3 was shown to be inactive. This ganglioside was dominating in adult pig. The apparent developmental disappearance of N-glycolyl groups in glycolipids of intestinal mucosa may have a correspondence in protein-linked sequences as well and thus explain the resistance of adult pigs to infection with E. coli K99.

【 授权许可】

Unknown