Functional expression of Ca2+ ‐mobilizing α‐thrombin receptors in mRNA‐injected Xenopus oocytes

FEBS Letters

Chambard, Jean Claude¹  Nargeot, Joel²  Lory, Philippe²  Pouysségur, Jacques¹  Van Obberghen-Schilling, Ellen¹ 

[1] Centre de Biochimie, C.N.R.S., Parc Valrose, 06034 Nice, France;Centre de Recherche de Biochimie Macromoléculaire, C.N.R.S., Route de Mende, 34033 Montpellier, France

关键词: α-Thrombin receptor;    Ca2+ mobilization;    Ca2+ -activated Cl− channel;    G-protein coupled receptor;    (Xenopus oocyte);    CCH;    carbachol;    EGTA;    (ethylenebis(oxyethyleneni-trilo)) tetraacetic acid;    FGF;    fibroblast growth factor;    G-protein;    GTP-binding regulatory protein;    GTP-γ-S;    guanosine 5'-O-(3-thio-triphosphate);    Hepes;    4-(2-hydroxyethyl)l-piperazineethanesulfonic acid;    5HT;    5-hydroxytryptamine;    IP3;    D-myo-inositol 1;    4;    5-tri-phosphate;    MBS;    modified Earth's solution;    PPACK;    D-phe-nylalanyl-L-prolyl-L-arginine chloromethyl ketone;    TH;    α-thrombin;   

DOI  :  10.1016/0014-5793(90)80221-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

α-Thrombin (TH) initiates a program of intracellular events that lead to DNA replication in quiescent CCL39 Chinese hamster lung fibroblasts via membrane receptors that have yet to be characterized at a molecular level. Functional TH receptors were expressed in Xenopus laevis oocytes following injection of poly(A)⁺ RNA from TH-responsive CCL39 cells; their presence was demonstrated by TH-stimulated ⁴⁵Ca²⁺ efflux or Ca²⁺dependent Cl⁻ channel activation. In voltage clamp experiments on microinjected oocytes a Ca²⁺ -activated Cl⁻ current was detected in response to TH (0.2–10 ). The TH response was blocked by a specific TH inhibitor, and potentiated by addition of FGF or intracellular injection of GTP-γ-S.

【 授权许可】

Unknown