Evidence for the convergence of β‐adrenergic and muscarinic signalling systems at a post‐receptor site

FEBS Letters

Ambudkar, Indu S.¹  Horn, Valerie J.¹  Baum, Bruce J.¹ 

[1] Clinical Investigations and Patient Care Branch, National Institute of Dental Research, NIH, Building 10, Room 1N-113, Bethesda, MD 20892, USA

关键词: Ca2+ mobilization;    Cyclic AMP;    Parotid;    Receptor crosstalk;    Atropine;   

DOI  :  10.1016/0014-5793(89)81604-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The β-adrenergic agonist isoproterenol stimulates inositol trisphosphate (IP₃) formation and cytosolic Ca²⁺ ([Ca²⁺]_i) mobilization in rat parotid acini via a cAMP-dependent process. Atropine, a muscarinic antagonist, inhibited these isoproterenol responses without affecting isoproterenol-induced amylase secretion or peak [Ca²⁺]_i and IP₃ responses elicited by α₁-adrenergic stimulation with epinephrine. Atropine had no effect on isoproterenol-induced [Ca²⁺]_i responses in a cell line which lacked muscarinic receptors and did not alter β-adrenoreceptor ligand binding. These results suggest that the inhibition by atropine results from a post-receptor effect on cAMP-mediated stimulation of phosphatidylinositol 4,5 bisphosphate (PIP₂) hydrolysis.

【 授权许可】

Unknown