Characterization of N‐cadherin mRNA in chicken brain and heart by means of oligonucleotide probes

FEBS Letters

Bock, Elisabeth¹  Dalseg, Anne-Marie¹  Andersson, Anna-Maria¹ 

[1]Research Center of Medical Biotechnology, University of Copenhagen, Protein Laboratory, 34 Sigurdsgade, DK-2200 Copenhagen N, Denmark

关键词: N-Cadherin;    Gene expression;    mRNA;    Chicken;    SDS-PAGE;    sodium dodecylsulphate polyacrylamide gel electrophoresis;    E10;    embryonal day 10;    P0 and P10;    postnatal day 0 and 10;   

DOI  :  10.1016/0014-5793(90)80198-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

It has previously been reported, that there is only one mRNA of 4.3 kb for chicken N-cadherin in brain and heart and one gene [(1988) J. Cell Biol. 106, 873-881]. Using three synthetic oligonucleotide probes derived from the published chicken N-cadherin cDNA sequence we found hybridization to at least four mRNAs in chicken brain and heart. The measured sizes were 8.0, 4.7, 3.8, and 3.3 kb. The 8.0 kb mRNA is only seen in brain, and only when a cytoplasmic probe is employed. The 3.8 kb mRNA is only observed in heart.

【 授权许可】

Unknown