Identification of the Arg‐Gly‐Asp sequence in laminin A chain as a latent cell‐binding site being exposed in fragment P1

FEBS Letters

Aumailley, Monique¹  Deutzmann, Rainer²  Gerl, Martin¹  Sonnenberg, Amoud³  Timpl, Rupert¹ 

[1] Max-Planck-Institut für Biochemie, D-8033 Martinsried, FRG;Department of Biochemistry, Microbiology and Genetics, University of Regensburg, Regensburg, FRG;Central Laboratory of the Netherlands Red Cross Blood Transfusion Service and Laboratory for Experimental and Clinical Immunology, University of Amsterdam, Amsterdam, The Netherlands

关键词: Basement membrane;    Cell attachment;    Integrin;    Proteolytic fragment;    Synthetic peptide;    EGF;    epidermal growth factor;    EHS;    Engelbreth-Holm-Swarm tumor;    HPLC;    high performance liquid chromatography;    TLCK;    tosyl lysyl chloromethane;   

DOI  :  10.1016/0014-5793(90)80159-G

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A single RGD-containing sequence present within an epidermal growth factor-like repeat of the short arms of laminin is shown by peptide inhibition to block integrin receptors recognizing a latent cell-binding site of laminin. Based on proteolysis data it is proposed that masking occurs by folding of the globular domain IVa over the cell-binding site in the adjacent rod-like structures of laminin A chain.

【 授权许可】

Unknown