Binding characteristics of complementary fibronectin fragments on artificial substrata

FEBS Letters

Kaetzel, Charlotte S.²  Zardi, Luciano³  Culp, Lloyd A.¹  Lewandowska, Kristine¹ 

[1] Department of Molecular Biology and Microbiology, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA;Department of Pathology, Case Western Reserve University, School of Medicine, Cleveland, OH 44106, USA;Cell Biology Laboratory, Istituto Nazionale per la Ricerca sul Cancro, 16132 Genoa, Italy

关键词: Fibronectin;    Proteolytic fragment;    Substratum binding;    Cell adhesion;    ELISA;   

DOI  :  10.1016/0014-5793(88)80166-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Various properties have been evaluated for the binding to tissue culture substrata of proteolytic fragments of human plasma or cellular fibronectins containing complementary sequences from the individual and alternatively spliced chains, since related fragments are known to yield differing adhesive responses from cells. These studies utilize ELISA methods and a polyclonal antiserum directed to human pFN for direct measurement or an occupancy test utilizing anti-albumin. Very related fragments (with or without an extra type III homology unit or extra domain_{a or b}) have significantly different properties in substratum binding and such differences provide a partial explanation for alteration of cellular adhesive responses on such fragments.

【 授权许可】

Unknown