Sphingomyelin is synthesized in the cis Golgi

FEBS Letters

Richard Schmidt, R.²  Karrenbauer, Achim¹  Jeckel, Dieter¹  Birk, Rolf²  Wieland, Felix¹ 

[1] Institut für Biochemie I, Im Neuenheimer Feld 328,6900 Heidelberg, FRG;Organische Chemie der Universität Konstanz, Universitätsstr. 10, 7750 Konstanz, FRG

关键词: Sphingomyelin biosynthesis;    Golgi subcompartment;    TLC;    thin layer chromatography;    t-CA;    truncated ceramide C8C8;    SPH;    sphingomyelin;    t-SPH;    truncated sphingomyelin C8C8;    CA;    ceramide;    ER;    endoplasmic reticulum;    TBS;    Trisbuffered saline pH 7.4;   

DOI  :  10.1016/0014-5793(90)80659-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have employed in vitro a truncated ceramide analogue with 8 carbon atoms in the sphingosine and the fatty acyl residue, each, to investigate the activity of various membrane fractions to synthesize truncated sphingomyelin. This shortened ceramide readily diffuses through membranes and therefore can easily find access to the lumina of intact organelles. Sphingomyelin synthase activity resides in the Golgi apparatus, and after sucrose density gradient centrifugation of Golgi-enriched fractions sphingomyelin synthesis follows a cis Golgi marker enzyme.

【 授权许可】

Unknown