Glucuronidation of bile acids in human liver, intestine and kidney

FEBS Letters

Infante, R.²  Salvat, C.²  Parquet, M.²  Pessah, M.²  Sacquet, E.¹  Raizman, A.² 

[1] Laboratoire d'Ecologie Microbienne, CNRZ-INRA, 78350 Jouy en Josas, France;INSERM Unité de Recherches d'Hépatologie U.9, Hôpital Saint-Antoine, 184 rue du Fg Saint-Antoine, 75571 Paris Cedex 12 France

关键词: Liver;    Kidney;    Intestine;    Bile acid;    Hyodeoxycholic acid;    tUDP-glucuronyl transferase;    Abbreviations: HDC;    hyodeoxycholic acid;    UDP-GT;    uridine diphosphate glucuronyl transferase;    GLC;    gas liquid chromatography;    TLC;    thin layer chromatography;   

DOI  :  10.1016/0014-5793(85)81020-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The activities of UDP-glucuronyl transferase(s) in homogenates and microsomal preparations of human liver, kidney and intestine were tested with hyodeoxycholic acid (HDC). The various kinetic parameters of the UDC-glucuronidation were determined from time course experiments. In both liver and kidney preparations, HDC underwent a very active metabolic transformation: liver K_m = 78 μM, V_max = 3.3 nmol·min ⁻¹mg ⁻¹ protein; kidney K_m = 186 μM, V_max = 9.9 nmol·min ^−1.mg ⁻¹ protein. To our knowledge this is the first observation of both an extensive and comparable bile acid glucuronidation occurring in renal and hepatic tissues.

【 授权许可】

Unknown