A new phosphoglycerolipid, ‘phosphatidylglucose’, found in human cord red cells by multi‐reactive monoclonal anti‐i cold agglutinin, mAb GL‐1/GL‐2

FEBS Letters

Nagatsuka, Yasuko²  Uzawa, Jun⁴  Hirabayashi, Yoshio⁵  Ono, Yasushi²  Shimizu, Kazufumi²  Ohashi, Yoko³  Kasama, Takeshi¹ 

[1] Laboratory of Biochemical Analysis, Tokyo Medical and Dental University, Tokyo, Japan;Department of Immunology and Microbiology, Nihon University School of Medicine, 30-1 Oyaguchikami-machi, Itabashi-ku, Tokyo 173-8610, Japan;Frontier Research Program, The Institute of Physical and Chemical Research, Tokyo, Japan;Division of Molecular Characterisation, The Institute of Physical and Chemical Research, Tokyo, Japan;Brain Science Institute, The Institute of Physical and Chemical Research, Tokyo, Japan

关键词: Epstein–Barr virus;    Human monoclonal antibody;    Cold agglutinin;    Cord red cell;    Glycolipid;    EBV;    Epstein–Barr virus;    PLA;    C and D;    phospholipase A;    C and D;    PI-PLC;    phosphatidylinositol-specific phospholipase C;    TLC;    thin layer chromatography;    SIMS;    secondary ion mass spectrum;    CID;    collision-induced dissociation;    TOCSY;    total correlation spectroscopy;   

DOI  :  10.1016/S0014-5793(01)02464-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cord red cell membranes express many differentiation-related molecules. To study such molecules, we have established human cell lines, termed GL-1 and GL-2, by the Epstein–Barr virus transformation method, both of which produce monoclonal anti-i cold agglutinin [Y. Nagatsuka et al., Immunol. Lett. 46 (1995) 93–100]. Thin layer chromatography immunoblotting analysis revealed that these antibodies had broad specificities reacting with a variety of glycolipid antigens. Of the immunoreactive lipid antigens, a new phosphoglycerolipid containing glucose from human cord red cells was found. The isolated lipid was unstable to alkaline hydrolysis and contained glucose as a sole sugar. Secondary ion mass spectrum–collision-induced dissociation mass spectrometric analysis of this lipid gave the main molecular ion peak at m/z 885 corresponding to phosphatidylhexose. This antigen was susceptible to phospholipases A2, C and D but resistant to phosphatidylinositol-specific phospholipase C. Two-dimensional nuclear magnetic resonance spectroscopy confirmed that glucose is linked to the sn-glycerol 3-phosphate residue with a β-anomeric configuration. Based upon these combined results, we identified this lipid as phosphatidyl-β-D-glucose. This is the first report showing the presence of the glucosylated glycerophospholipid in mammalian sources.

【 授权许可】

Unknown