Amplification of the Na+‐ATPase of Streptococcus faecalis at alkaline pH

FEBS Letters

Igarashi, Kazuei¹  Kakinuma, Yoshimi¹ 

[1] Faculty of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi, Chiba 260, Japan

关键词: ATPase;    Na+-;    Induction;    Alkaline pH;    Proton potential;    (Streptococcus faecalis);    PMSF;    phenylmethylsulfonyl fluoride;    DCCD;    dicyclohexylcarbodiimide;    TCS;    tetrachlorosalicylanilide;   

DOI  :  10.1016/0014-5793(90)80654-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Na⁺-ATPase activity of Streptococcus faecalis was influenced by the medium pH. Activities of the protonophore-resistant Na⁺ extrusion and the KtrII (active K⁺ uptake by the Na⁺-ATPase) were maximal in the cells grown at pH 9.5, and were minimal in those grown at pH 6.O. In the cells grown at pH 7.5, they were moderately observed. The Na⁺-stimulated ATPase activity of the cells grown at pH 9.5 was about 4-fold higher than that of the cells grown at pH 6.O. Thus, amplification of the Na⁺-ATPase is remarkable at alkaline pH in this organism, possibly by an increase of the cytoplasmic Na⁺ level as a signal.

【 授权许可】

Unknown