Stimulation of nerve growth factor synthesis/secretion by 1,4‐benzoquinone and its derivatives in cultured mouse astroglial cells

FEBS Letters

Furukawa, Yoshiko²  Furukawa, Shoei¹  Hayashi, Kyozo²  Takeuchi, Rie²  Murase, Katsuhito² 

[1] Division of Immunology, National Center of Neurology and Psychiatry, Ogawa-Higashi 4-1-1, Kodaira, Tokyo 187, Japan;Department of Pharmaceutics, Gifu Pharmaceutical University, Mitahora-Higashi 5-6-1, Gifu 502 Japan

关键词: Nerve growth factor;    1;    4-Benzoquinone;    Coenzyme Qo;    Vitamin K3;    (Astroglial cell);    NGF;    nerve growth factor;    EIA;    enzyme immunoassay;    BSA;    bovine serum albumin;    FCS;    fetal calf serum;    DMEM;    Dulbecco's modified Eagle's medium;    CM;    conditioned medium;   

DOI  :  10.1016/0014-5793(90)80637-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Previously we reported that astroglial cells cultured from mouse brain synthesize and secrete nerve growth factor (NGF) and that, in quiescent cells, catecholamines markedly increase the NGF content in the conditioned medium (CM). We wished to further assess the structural properties required for exhibition of such effect of compounds containing a ring structure analogous to that of catechol on astroglial NGF synthesis. During our study, we found that hydroquinone, which was confirmed not to stimulate NGF synthesis in mouse flbroblast cells in another of our investigations, is a potent stimulator of NGF synthesis in astroglial cells and that 1,4-benzoquinone, an oxidized form of hydroquinone, is a more effective stimulator than hydroquinone itself. In addition, the results of experiments with 1,2-benzoquinone derivatives indicated that the presence of a long aliphatic side chain in the molecule eliminates the stimulatory effect of 1,4-benzoquinone on NGF synthesis in astroglial cells.

【 授权许可】

Unknown