Nerve growth factor induces mast cell degranulation without changing intracellular calcium levels

FEBS Letters

Otten, U.¹  Weskamp, G.¹  Mazurek, N.¹  Erne, P.² 

[1] Departments of Biophysical Chemistry and Pharmacology, Biocenter, University of Basel, CH-4056 BaselSwitzerland;Departments of Biophysical Chemistry and Kantonsspital Basel, CH-4031 Basel, Switzerland

关键词: Nerve growth factor;    12-O-Tetradecanoylphorbol 13-acetate;    (Rat peritoneal mast cell;    Rat leukemia cell line);    Degranulation;    Ca2+;   

DOI  :  10.1016/0014-5793(86)80428-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Nerve growth factor (NGF) induces degranulation of rat peritoneal mast cells (RPMC) in a dose-dependent manner, providing direct evidence for its action on non-neuronal tissues. Activation of RPMC by NGF depends on lysophosphatidylserine and extracellular calcium. NGF-mediated RPMC degranulation is not coupled to a transient increase in intracellular free calcium ([Ca²⁺]). It is suggested that NGF has a unique mode of action independent of ([Ca²⁺])_i and presumably also without involving protein kinase C activation as indicated by the effects of phorbol esters and NGF on antigen-evoked [Ca²⁺]_i rise.

【 授权许可】

Unknown