The influence of nerve growth factor on the activities of adenylate cyclase and high‐affinity GTPase in pheochromocytoma PC12 cells

FEBS Letters

Severin, E.S.¹  Golubeva, E.E.¹  Kondratyev, A.D.¹  Melnik, E.I.¹  Posypanova, G.A.¹ 

[1] Institute of Applied Molecular Biology, USSR Ministry of Health, Sympheropolsky Blvd, 8, Moscow 113149, USSR

关键词: Nerve growth factor;    Adenylate cyclase;    GTPase;    (PC12 cell);   

DOI  :  10.1016/0014-5793(89)81341-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The influence of nerve growth factor (NGF) on the activities of adenylate cyclase and high-affinity GTPase in pheochromocytoma PC12 cells was studied. Incubation of cells with nerve growth factor led to a rapid activation of adenylate cyclase accompanied by an inhibition of high-affinity GTPase. By the 10th min of incubation the activity of adenylate cyclase had been reduced 2-fold when compared to the control. The activity of GTPase, however, increased. No significant changes in the cAMP level were detected. The data obtained indicate that NGF interaction with PC12 cells induces changes in the adenylate cyclase system and this process involves G-proteins that regulate the adenylate cyclase activity.

【 授权许可】

Unknown