Sequence analysis of the promoter region of the rat vasopressin gene

FEBS Letters

Mohr, Evita¹  Richter, Dietmar¹ 

[1] Institut für Zellbiochemie und Klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Martinistr. 52, 2000 Hamburg 20, FRG

关键词: Vasopressin gene expression;    Glucocorticoid response element;    Binding site;    AP2;    cyclic AMP response element;    Gel shift assay;    Nuclear protein fraction;   

DOI  :  10.1016/0014-5793(90)80130-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The vasopressin gene is highly transcribed in magnocellular neurons of the supraoptic (SON) and paraventricular nucleus (PVN) in the rat hypothalamus. In order to identify cis-acting elements involved in the expression of the vasopressin gene, approximately 1 kb upstream of the transcription start site has been sequenced. Several putative regulatory elements have been detected, including a glucocorticoid response element (GRE), a cAMP response element (CRE), and four AP2 binding sites. In gel shift assays performed with a labelled DNA fragment corresponding to nucleotide residues −214 to −36 and nuclear proteins extracted from SON-derived tissue enriched in magnocellular neurons, three specific protein-DNA complexes have been detected. Complex formation is effectively competed by addition of an excess of unlabelled fragment.

【 授权许可】

Unknown