FEBS Letters | |
Sequence analysis of the promoter region of the rat vasopressin gene | |
Mohr, Evita1  Richter, Dietmar1  | |
[1] Institut für Zellbiochemie und Klinische Neurobiologie, Universitäts-Krankenhaus Eppendorf, Martinistr. 52, 2000 Hamburg 20, FRG | |
关键词: Vasopressin gene expression; Glucocorticoid response element; Binding site; AP2; cyclic AMP response element; Gel shift assay; Nuclear protein fraction; | |
DOI : 10.1016/0014-5793(90)80130-B | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The vasopressin gene is highly transcribed in magnocellular neurons of the supraoptic (SON) and paraventricular nucleus (PVN) in the rat hypothalamus. In order to identify cis-acting elements involved in the expression of the vasopressin gene, approximately 1 kb upstream of the transcription start site has been sequenced. Several putative regulatory elements have been detected, including a glucocorticoid response element (GRE), a cAMP response element (CRE), and four AP2 binding sites. In gel shift assays performed with a labelled DNA fragment corresponding to nucleotide residues −214 to −36 and nuclear proteins extracted from SON-derived tissue enriched in magnocellular neurons, three specific protein-DNA complexes have been detected. Complex formation is effectively competed by addition of an excess of unlabelled fragment.
【 授权许可】
Unknown
【 预 览 】
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RO201912020292997ZK.pdf | 466KB | download |