Vasopressin gene expression is stimulated by cyclic AMP in homologous and heterologous expression systems

FEBS Letters

Burbach, J.Peter H.¹  Verbeeck, Marleen A.E.¹  Adan, Roger A.H.¹ 

[1]Rudolf Magnus Institute, Medical Faculty, University of Utrecht, Vondellaan 6, 3521 GD Utrecht, The Netherlands

关键词: Vasopressin gene expression;    Gene regulation;    cyclic AMP;    Small cell lung carcinoma cell line;    AP-2;    cAMP;    cyclic AMP;    VP;    vasopressin;    SON;    supraoptic;    PVN;    paraventricular;    ec;    embryonal carcinoma;    SCLC;    small cell lung carcinoma;    IBMX;    isobutyl-methylxanthine;    8;    Br-cAMP;    8;    Bromo-cAMP;   

DOI  :  10.1016/0014-5793(90)80455-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The possible role of cyclic AMP (cAMP) in the regulation of the vasopressin (VP) gene was tested in two cellular expression systems: one cell line with endogenous VP expression and the other which was transiently with a VP promoter-luciferase fusion gene. 8, Bromo-cAMP stimulated the VP mRNA content about 4-fold in the human VP-expressing small cell lung carcinoma cell line GLC-8. The luciferase activity in P19 embryonal carcinoma cells which were transiently transfected with − 174 to +44 of the 5'-flanking region of the human VP gene linked to the firefly luciferase gene, was stimulated about 2-fold by the cAMP analogue. The results indicate that cAMP plays a role in the upregulation of the VP gene and hence point to several putative nucleotide motives in the promoter functionally conferring this response.

【 授权许可】

Unknown