Identification of an interleukin‐1 beta binding protein in human plasma

FEBS Letters

Eastgate, J.A.¹  Duff, G.W.¹  Symons, J.A.¹ 

[1] University of Edinburgh Department of Medicine, Molecular Immunology Group, RDU, Northern General Hospital, Ferry Road, Edinburgh EH5 2DQ, Scotland

关键词: Interleukin 1 alpha;    Interleukin 1 beta;    Soluble binding protein;    Interleukin 1 receptor;   

DOI  :  10.1016/0014-5793(90)80106-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A covalent cross-linking technique was used to bind iodinated interleukin-1 (IL1) alpha and beta to plasma proteins. One specific IL1 beta binding protein was observed, that when cross-linked to ¹²⁵I-ILl beta migrated to approximately 60 kDa on SDS-PAGE. The protein did not bind IL1 alpha. The 43 -kDa protein was partially purified using a wheat germ agglutinin affinity column. The isolated factor again specifically bound IL1 beta, and appeared to consist of single chain glycoprotein. The protein was heat stable and had a rapid association time with IL1 beta. This protein may be an important carrier molecule for IL1 beta in vivo.

【 授权许可】

Unknown