Heterogeneity of protein kinase C expression and regulation in T lymphocytes

FEBS Letters

Marais, R.²  Cantrell, D.A.³  Graves, J.D.³  Lucas, S.³  Alexandere, D.¹  Parker, P.² 

[1] Cell Surface Biochemistry Laboratory, Imperial Cancer Research Fund, Lincoln's Inn Fields, London WC2A 3PX, England;Ludwig Institute for Cancer Research, Middlesex Hospital, London W1P 8BT, England;Lymphocyte Activation Laboratory, Imperial Cancer Research Fund. Lincoln's Inn Fields, London WC2A 3PX, England

关键词: Protein kinase C;    Signal transduction;    CD3 antigen;    PDBu;    phorbol 12;    13-dibutyrate;    PKC;    protein kinase C;    IN2;    interleukin 2;    SDS;    sodium dodecyl sulphate;   

DOI  :  10.1016/0014-5793(90)80064-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The purpose of the present study was to examine protein kinase C (PKC) isotype expression in T lymphoblasts derived from peripheral blood and the T leukaemic cell Jurkat. Using antisera reactive with PKC α,β₁, and β₂ and γ, it was observed that T cells expressed two PKC isotypes, PKC α and β. No PKC γ was detected in T lymphocytes. In lymphoblasts, high levels of PKC β compared to PKC α were found whereas Jurkat cells expressed high levels of α compared to PKC β. Differences in the calcium sensitivity of phorbol ester-induced phosphorylation were observed in Jurkat and T lymphoblasts which correlated with the relative levels of PKC α and β isotypes expressed by the cells.

【 授权许可】

Unknown