Rapid myosin phosphorylation transients in phasic contractions in chicken gizzard smooth muscle

FEBS Letters

Pfitzer, G.¹  Fischer, W.¹ 

[1] II. Physiologisches Institut, Universität Heidelberg, D-6900 Heidelberg, FRG

关键词: Smooth muscle;    Myosin light chain phosphorylation;    Smooth muscle mechanics;    (Chicken gizzard;    intact);    TCA;    trichloroacetic acid;    Hepes;    N-2-hydroxyethyl-piperazine-N'-2-ethanesulfonic acid;    MLCK;    myosin light chain kinase;   

DOI  :  10.1016/0014-5793(89)81615-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In intact smooth muscle strips from chicken gizzard, electrical stimulation and carbachol elicited brief, phasic contractions which were associated with a very rapid, transient phosphorylation of the 20 kDa myosin light chains. The phosphorylation transients reached their peak after 3 s and 6 s and preceded that of force. Phosphorylation was not significantly different from basal levels after 10 s and 30 s while force still amounted to 50% of the peak value. The rate of tension decline could be increased by cessation of stimulation or by addition of atropine, even at apparently basal phosphorylation levels suggesting a phosphorylation independent regulation.

【 授权许可】

Unknown