FEBS Letters | |
Rapid myosin phosphorylation transients in phasic contractions in chicken gizzard smooth muscle | |
Pfitzer, G.1  Fischer, W.1  | |
[1] II. Physiologisches Institut, Universität Heidelberg, D-6900 Heidelberg, FRG | |
关键词: Smooth muscle; Myosin light chain phosphorylation; Smooth muscle mechanics; (Chicken gizzard; intact); TCA; trichloroacetic acid; Hepes; N-2-hydroxyethyl-piperazine-N'-2-ethanesulfonic acid; MLCK; myosin light chain kinase; | |
DOI : 10.1016/0014-5793(89)81615-1 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
In intact smooth muscle strips from chicken gizzard, electrical stimulation and carbachol elicited brief, phasic contractions which were associated with a very rapid, transient phosphorylation of the 20 kDa myosin light chains. The phosphorylation transients reached their peak after 3 s and 6 s and preceded that of force. Phosphorylation was not significantly different from basal levels after 10 s and 30 s while force still amounted to 50% of the peak value. The rate of tension decline could be increased by cessation of stimulation or by addition of atropine, even at apparently basal phosphorylation levels suggesting a phosphorylation independent regulation.
【 授权许可】
Unknown
【 预 览 】
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