Mildly oxidised low density lipoprotein induces platelet shape change via Rho‐kinase‐dependent phosphorylation of myosin light chain and moesin

FEBS Letters

Retzer, Michaela¹  Essler, Markus¹  Siess, Wolfgang¹ 

[1] Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Pettenkoferstr. 9, 80336 Munich, Germany

关键词: Platelet shape change;    Rho-GTPase;    Oxidized low density lipoprotein;    Rho-kinase;    Myosin light chain phosphorylation;    Moesin phosphorylation;    C3;    C3-transferase from Clostridium botulinum;    mox-LDL;    mildly oxidised low density lipoprotein;    LPA;    lysophosphatidic acid;    MLC;    myosin light chain;    MLCK;    MLC-kinase;   

DOI  :  10.1016/S0014-5793(99)01762-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oxidised low density lipoprotein (LDL) plays an important role in the pathogenesis of atherosclerosis. Here we demonstrate that mildly oxidised (mox) LDL engages the GTPase Rho and its effector molecule p160 Rho-kinase to induce phosphorylation of myosin light chain and of moesin leading to platelet shape change. Pretreatment of platelets with the selective Rho inhibitor C3-transferase from Clostridium botulinum or with the Rho-kinase inhibitor Y-27632 blocked mox-LDL-induced myosin light chain phosphorylation, moesin phosphorylation and shape change. Mox-LDL did not induce an increase in cytosolic Ca²⁺ during shape change. We propose that Rho/Rho-kinase inhibition could be a strategy for prevention of the pathologic platelet activation during atherogenesis.

【 授权许可】

Unknown