Metabolism of peripheral lymphocytes, interleukin‐2‐activated lymphocytes and tumor‐infiltrating lymphocytes from 31 NMR studies

FEBS Letters

Cohen, Jack S.¹  Kaplan, Ofer¹  Aebersold, Paul² 

[1] Medicine Branches, Division of Cancer Treatment, National Cancer Institute, NIH, Bethesda, MD 20892, USA;Surgery Branches, Division of Cancer Treatment, National Cancer Institute, NIH, Bethesda, MD 20892, USA

关键词: Lymphocyte;    Nuclear magnetic resonance;    31P;    Interleukin-2;    Lymphocyte;    tumor-infiltrating;    Lymphocyte;    IL-2 activated;    Metabolism;   

DOI  :  10.1016/0014-5793(89)81614-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

³¹P NMR spectra of tumor-infiltrating lymphocytes (TILs) were found to be significantly different from those of normal peripheral lymphocytes. The greatest difference was in the phosphodiester (PDE) region, mainly in the glycerophosphocholine (GPC) signal. Short-term activation of peripheral lymphocytes with interieukin-2 induced a small increase in ATP levels. In all lymphocytes the phosphomonoester (PME) region is dominated by phosphoethanolamine (PE), while there is an unusual absence of phosphocholine (PC). Perfusion of these cells with high concentrations of choline caused only a minimal increase in PC, indicating that choline kinase is not the rate limiting step of lecithin synthesis in lymphocytes.

【 授权许可】

Unknown